Fecal black egg nest|150765 |BNCC

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Cyathus stercoreus

Subculture procedure	① Prepare one 90mm solid Agar plate, one inoculation shovel, and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, cultured on a comprehensive PDA medium, have distinct colonies, white hyphae, dense and flat, and spread towards the edge of the agar plate
Sharing mode	Public welfare sharing

Cyathus stercoreus

Storage conditions : 2~8 ℃

No. 150765

product form:agar slant in 14mm test tube

Validity: growing culture, in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be regarded as good receiving goods. The inclined plane of the test tube can be used directly. in principle, it can be used many times without pollution during the storage period, but its vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, put into distilled water and boil for 30min, take the filtrate to 1000mL for later use. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5-7days strain layer become obvious
colony morphology:	small filamentous fungi, cultured on integrated PDA medium, the colonies were obvious, the hyphae were white, the hyphae were densely flat, spread to the edge of the plate, and the back of the medium was light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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