Chromogenic Streptomyces|152765 |BNCC

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Streptomyces chromogenes

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 24-48 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Round, irregular edges, opaque, flat shape, rough surface, wrinkled surface, no pigment production, G+(blue purple), Bacillus, purity: pure, dry texture, difficult to pick/cut, gray white front, light color
Sharing mode	Public welfare sharing

Streptomyces chromogenes

Storage conditions: 2~8 ℃

No. 152765

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, Gao's No. 1 medium, 24-48h. Gao's No. 1 medium: soluble starch 20.0g, sodium chloride 0.5g, ferrous sulfate 0.01g, potassium nitrate 1.0g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, agar 15g, distilled water 1.0L, pH 7.3±0.2. Sterilize at 121℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 48h plate bacteria layer is obvious;colony is typical on the streaked plate,
colony morphology:	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white uplift: flat surface: rough texture: dry and difficult to pick
Conclusion:	good viability, no abnormal colony morphology, qualified

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