Streptomyces prasinus

Storage conditions : 2~8 ℃

No. : 152874

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:  28 ℃, aerobic, Goblet synthesis No. 1 agar, 3-5 days. Gao's Synthesis No. 1 Agar: 20g of soluble starch, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 1.0g of potassium nitrate, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 15g of agar, 1.0L of distilled water, pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Place all the plates under the above culture conditions, and the strains can be used when they grow out.  

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows