Thermophilic Bacillus subtilis|186178 |BNCC

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Geobacillus stearothermophilus

Culture medium	BNCC363070
Description	Tryptic Soy Agar (TSA)
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	55 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1–2 mm, circular, regular margin, opaque, grayish-white on the obverse, convex in the center, smooth and shiny surface, moist texture, easily picked, Gram‑positive (bluish‑purple), bacillus, purity: pure.
Sharing mode	Public welfare sharing