Thermophilic Bacillus subtilis|186178 |BNCC

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Geobacillus stearothermophilus

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	55 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1–2 mm, circular, regular margin, opaque, grayish-white on the obverse, convex in the center, smooth and shiny surface, moist texture, easily picked, Gram‑positive (bluish‑purple), bacillus, purity: pure.
Sharing mode	Public welfare sharing

1. Description

1. Name: Geobacillus stearothermophilus

2. BNCC No.: 186178

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions

1, Tryptone soybean agar (TSA): tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean meal papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,pH7.3±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 55 ℃

4. Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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