Clostridium sporogenes|186188 |BNCC

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Clostridium sporogenes

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 2-3 days; anaerobic;
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1–2 mm, circular, irregular margin, opaque, grayish-white on the obverse, convex in the center, smooth and shiny surface, moist texture, easily picked, Gram‑positive (bluish‑purple), bacillus, purity: pure.
Sharing mode	Public welfare sharing