Clostridium butyricum|186498 |BNCC

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Clostridium butyricum

Subculture procedure	Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After thorough dissolution, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The diameter of the colony is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing

Clostridium butyricum

Storage conditions: 2~8 ℃

No. 186498

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, anaerobic, Columbia blood plate (ready to use), 24-48h.

Recovery steps:

①Prepare 1-2 of above mentioned plates(Place it in an anaerobic environment for deoxygenation for 24h in advance);

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 24-48h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48hours strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque color: off-white ; Protuberance: middle bulge; Surface: bright and smooth ; Texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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