Mycobacterium genus|191574 |BNCC

Essential Information References Certificate Related Products

Mycobacterium sp.

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	30 ℃; 48-72 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Orange Elevation: Central dome Surface: Bright and smooth Texture: Moist and viscous
Sharing mode	Public welfare sharing

Mycobacterium sp.

No. 191574

Storage conditions: 2~8 ℃

Product format: freeze dried 200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: Level 1, ultra-clean desk or safety cabinet operation

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, nutritious gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121℃,15min

Recovery steps:

(1)Prepare a flask of liquid media or two agar plates.

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 30℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a agar plate evenly, then put the plates in incubator at 30℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 24h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology:	size: 1-2mm color: yellow shape: round edge: neat wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

inspector/date: boffei 2019.11.15 auditor signature: Sudan

Download certificate