Alabama Aspergillus|277447 |BNCC

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Aspergillus alabamensis Balajee et al.

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on PDA medium, white hyphae, flat colonies, spreading growth, producing water-soluble orange pigments, and the back of the medium is orange red
Sharing mode	Public welfare sharing

Aspergillus alabamensis

Storage conditions: 2~8 ℃

No. 277447

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,3-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare sterile water and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, dissolve it fully and mix well.

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 3 days plate colonies are obvious
colony morphology: (above)	small filamentous fungi have obvious colonies on PDA medium, hyphae are white, colonies are flat, spread and grow, producing water-soluble orange pigment, and the back of the medium is orange-red.
Conclusion	good viability, no abnormal colony morphology, qualified

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