Aspergillus|230081 |BNCC

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Mortierella sp.

Subculture procedure	① Prepare 1-2 comprehensive PDA plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious white colonies and dense and vigorous hyphae on comprehensive PDA medium, spreading and growing towards the edge of the agar plate,
Sharing mode	Public welfare sharing

1.Description

1. Name： Boletus edulis

2. BNCC No.：230081

3. Biosafety level： 4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA: 1000mL of potato juice, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate, 20g of glucose, 10mg of vitamin B1, 20g of agar, and natural pH. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature：25 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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