BeNa Culture Collection
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| Subculture procedure | ① Prepare 2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 28 ℃; 5-7 days; aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | In the early stage, white mycelium grows, and in the later stage of cultivation, the mycelium begins to produce black spores |
| Sharing mode | Public welfare sharing |
Aspergillus brasiliensis
Storage conditions: 2~8 ℃
No. 286403
Product format :freeze dried,200ul
Validity period: 6 years
Biosafety level: 1, handle in ultra-clear table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:28 ℃, aerobic, 5-7 days, comprehensive PDA agar medium: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability，in 3 days floc grows at the top of the culture solution, and the plate bacteria layer is obvious.In 5 days strain layer become obvious, colony is typical on the streaked plate |
|
Colony morphology (pictured above) |
filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae, dense/low flat, The hyphae spread vigorously and produced a large number of black spores. |
| Conclusion | good viability, no abnormal colony morphology, qualified |
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