Streptococcus parahaemolyticus|317937 |BNCC

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Streptococcus parasanguinis Whiley et al.

Subculture procedure	Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, gray on the surface, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing

Streptococcus parasanguinis

Storage conditions: 2~8 ℃

No. 317937

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, anaerobic, modified GAM agar, 48-72h, modified GAM agar: peptone 15.0g, tryptone 10.0g, soy peptone 3.0g, yeast extract 5.0g, beef powder 2.0g, beef liver extract 1.2g, soluble starch 0.3g, L-cysteine 0.15g, glucose 3.0g, potassium dihydrogen phosphate 2.5g, digestive serum powder 13.5g, sodium thioglycolate 0.15g, sodium chloride 3.0g, agar 13.0g (not included in liquid medium), distilled water: 1L, pH 7.0±0.2, 25℃

Additives: 0.02% vitamin K1 solution and hemin must be added

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability，in 3 days the liquid medium is turbid, and the plate bacterial layer is obvious; Bacterial liquid scribing plate, colony typical
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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