Brewing yeast|336026 |BNCC

Essential Information Certificate Related Products

Saccharomyces cerevisiae Hansen

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	30 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Saccharomycescerevisiae Hansen

Description

1. BNCC No.: BNCC 336026

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2

2. Atmosphere: aerobic

3. Temperature: 25-28 ℃

Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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