Black fungus (purple fungus, yellow backed fungus)|336039 |BNCC

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Auricularia polytricha(Mont.)Sacc.

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 2-3 weeks; aerobic;
Subculture procedure	① Dissolve the frozen tube in a 30 ℃ water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus that spreads and grows on a comprehensive PDA medium. In the early stage, the hyphae are white, sparse, and radiating, and in the later stage, the hyphae gradually become dense and fluffy. The back of the medium is light yellow
Sharing mode	Public welfare sharing