Gray tree flower|336081 |BNCC

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Grifola frondosa(Fr.)S.F.Gray

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 3-5 days; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white and dense hyphae that spread and grow towards the edge of the agar plate. The back of the medium is light yellow
Sharing mode	Public welfare sharing