Aspergillus niger|336179 |BNCC

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Aspergillus niger van Tieghem

Subculture procedure	① Prepare 1-2 fresh plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Filamentous fungi have obvious colonies on malt extract agar, and the mycelium spreads and grows vigorously. In the early stage, the mycelium changes from white to yellow, and in the later stage, black spores are produced
Sharing mode	Public welfare sharing

1.Description

1. Name： Aspergillus niger van Tieghem

2. BNCC No.：336179

3.Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. 5 °Bé wort agar: 5 °Bé wort 1.0L, agar 15.0g, natural pH. 121 ℃,15min.

2. Atmosphere： aerobic

3. Temperature： 28-30 ℃

4.Notes：

1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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