Wanshi Penicillium|336231 |BNCC

Essential Information Certificate Related Products

Paecilomyces variotii

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Filamentous, producing yellow spores, yellow white on the front and yellow on the back
Sharing mode	Public welfare sharing

Paecilomyces variotii Bainier

Storage conditions : 2~8 ℃

No. : 336231

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Growth conditions :25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA:20% potato liquid 1.0L, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, vitamin B1 8mg, agar 20.0g,pH natural. 121 ℃,15min. 20% potato liquid configuration: take 200g of peeled potato, boil for 30min, filter to take filtrate, and make up to 1.0l.

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 6 days strain layer is obvious
colony morphology	small filamentous fungi have obvious colonies on the integrated PDA medium. the hyphae are white cotton wool-like at the beginning, dense and vigorous, and later produce yellow-green spores. the back of the medium is light yellowish brown.
conclusion	good viability, no abnormal colony morphology, qualified

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