Dark Ringed Charcoal Fungus|336334 |BNCC

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Annulohypoxylon stygium

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 5-7 days; aerobic;
Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have distinct colonies on a comprehensive PDA culture medium, with light yellow central hyphae, white edges, and fuzzy morphology, spreading and growing towards the edge of the agar plate. There are black ring patterns on the back of the culture medium
Sharing mode	Public welfare sharing