Lactic acid Kruve yeast|186194 |BNCC

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Kluyveromyces lactis

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 24-48 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, milky white on the front, opaque, protruding in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Kluyveromyces lactis

No.: 186194

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: freeze-dried tube 24 months

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice：if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, Shah glucose agar (SDA). SDA medium: glucose 40g, peptone (casein trypsin digest, gastric enzyme digest of animal tissue) 10g, agar 15g, distilled water 1.0L,pH 5.6±0.2,121 ℃, 15min sterilization.

Recovery steps:

(1) Prepare 1-2 pieces of SDA plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a SDA agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 40h SDA plate scribing colony is obvious
colony morphology:	size: general color: milky white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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