BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; aerobic |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80℃ |
| Safety level | 1 |
| morphology | Spread growth, filamentous, irregular edges, opaque, gray white on the front and gray white on the back, flat shape, rough surface, wrinkled surface, dry texture |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
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Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
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Mycoplasma fermentans DNA standard
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Acinetobacter baumannii nucleic acid reference (Heat inactivated) (Strongly positive)
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Candida tropicalis nucleic acid reference (Heat inactivated) (Strongly positive)
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Mycobacterium kansasii nucleic acid reference (Heat inactivated) (Strongly positive)
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Lactobacillus intestinalis DNA standard
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Enterococcus faecium DNA lab quality control
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