Vibrio parahaemolyticus (halophilic bacteria)|186296 |BNCC

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Vibrio parahaemolyticus

Culture medium	BNCC353940
Description	3.5% Sodium Chloride Agar Medium
Composition	Beef Extract:3.0g, Peptone:10.0g, NaCl:35.0g, Agar:15.0g, pH:7.2-7.4(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front and gray white on the back, protruding in the middle, smooth on the surface, moist texture, G - (red), Vibrio, purity: pure
Sharing mode	Public welfare sharing