Brazilian Aspergillus|337919 |BNCC

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Aspergillus brasiliensis

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	On the comprehensive PDA medium, the colonies are obvious, the hyphae are vigorous, spreading and growing, covering the Agar plate. The hyphae are white in the early stage, turn black in the later stage, produce black spores, and have a darker color on the reverse side
Sharing mode	Public welfare sharing

Description

1. Name: Aspergillus niger

2. BNCC No.:337919 ;

3. Biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere: aerobic

3.Temperature: 25-28 ℃

precautions

1. Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2. Agar slant shall be inoculated asap, and long-term storage is not recommended.

3. Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4. Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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