Clostridium butyricum|337239 |BNCC

Essential Information References Certificate Related Products

Clostridium butyricum

Subculture procedure	Draw lines on a flat Agar plate and prepare a liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified cultivation conditions mentioned above. If the bacterial solution is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions	37 ℃; 18-24h; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, protruding in the middle, rough surface, moist texture, easy to pick up, and rod-shaped
Sharing mode	Public welfare sharing

Clostridium butyricum

Storage conditions: 2~8 ℃

No. 337239

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Clostridium sporogenes medium, 48-72h. Clostridium sporogenes culture medium: yeast extract 3.0g, beef extract 10.0g, glucose 5.0g, tryptone 10.0g, sodium chloride 5.0g, soluble starch 1.0g, cystine hydrochloride 0.5g, sodium acetate 3.0g, agar 20g (not included in liquid medium),pH 6.8±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates(Put it in an anaerobic environment for deoxygenation for 24h in advance);

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 48-72h liquid medium become turbid, strain layer is obvious; colony is typical on marked plate
colony morphology: (above)	size: 1-2mm shape: round edge: irregular transparency: opaque color: white uplift: middle convex surface: bright texture: moist and viscous
conclusion	good viability, no abnormal colony morphology, qualified

Download certificate