Fusobacterium nucleatum subspecies|337404 |BNCC

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Fusobacterium nucleatum subsp nucleatum

Subculture procedure	Draw lines on a flat Agar plate and prepare a liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions	37 ℃; 48-72 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Fusobacterium nucleatum subsp nucleatum

2.BNCC No.:337404

3.Biosafety level: 3

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Culture medium: Columbia blood plate (finished product)

2.Atmosphere: anaerobic

3.Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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