BeNa Culture Collection
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| Culture medium | BNCC353538 |
| Description | Liquid thioglycolate culture medium |
| Composition | Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C2H3NaO2S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72 hours; anaerobic |
| Subculture procedure | Draw lines on a flat Agar plate and prepare a liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
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