Rhizopus oryzae|337563 |BNCC

Essential Information Certificate Related Products

Rhizomucor miehei

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spreading growth, initially white gradually turning grayish-black, filamentous, with yellow undersides
Sharing mode	Public welfare sharing

1.Description

1. Name: Rhizomucormiehei

2. BNCC No.:337563

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. CPDA medium: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃.

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

Download certificate