BeNa Culture Collection
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| Culture medium | BNCC352255 |
| Description | Streptomyces medium 2 |
| Composition | Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 30 ℃; 48-72 hours; aerobic |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, dark in color, raised in the middle, rough on the surface, wrinkled on the surface, hard in texture, difficult to pick/cut, does not produce pigments, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
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Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
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