Streptomyces avidans|337586 |BNCC

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Streptomyces avidinii

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	30 ℃; 48-72 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, dark in color, raised in the middle, rough on the surface, wrinkled on the surface, hard in texture, difficult to pick/cut, does not produce pigments, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces avidinii

2. BNCC No.:337586

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. ISP-2 medium: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 15.0g, distilled water 1.0 L,pH7.3. 121 ℃,15 minutes sterilization.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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