Mycobacterium bovis|339446 |BNCC

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Mycobacterium bovis Karlson and Lessel

Culture medium	BNCC375932
Description	Middlebrook 7H9 Broth
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.0g, Na₂HPO₄:2.5g, Na₃C₆H₅O₇:0.1g, MgSO₄:0.05g, CaCl₂:0.0005g, ZnSO₄:0.001g, CuSO₄:0.001g, L-Glutamic acid(C₅H₉NO₄):0.5g, C₆H₈FeNO₇:0.04g, Vitamin B6(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.0005g, ADC enrichment:10%(added at 50℃), pH:6.6±0.2(25℃)
Growth conditions	37 ° C; 3-4 weeks; aerobic
Subculture procedure	① Prepare the above two tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation, and the strain can be used once it grows (during the cultivation process, the agar plate needs to be kept moist, and 7H9 broth can be added).
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing