Hansenula yeast|186828 |BNCC

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Debaryomyces hansenii

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	30 ℃; 2-3 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	单菌落大小2-4mm，形状圆形，边缘整齐，不透明，颜色乳白。
Sharing mode	Public welfare sharing

Debaryomyces hansenii

Storage conditions : 2~8 ℃

No. : 186828

Product format : freeze dried,200ul

Validity period : freeze-dried tube 24 months

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:

(1) Prepare 1-2 pieces of YM plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a YM agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability, in 40h YM plate colony is obvious
colony morphology	size: medium color: milky white shape: round edge: neat and wet edge: wet and smooth: smooth and bright transparency: opaque bulge: bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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