Salmonella enteritis subspecies|340081 |BNCC

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Salmonella enterica subsp. enterica

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24 hours; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	G-bacteria Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey White Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Moist
Sharing mode	Public welfare sharing

Description

1. Name:Salmonella enterica subsp. enterica
2. BNCC No.:340081
3. Biosafety level:4
Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C
Growth Conditions
1. Nutritional gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production.
2. Atmosphere:aerobic
3. Temperature: 37 ℃

Notes:

1.Normal culturing time, 1-2 days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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