Staphylococcus aureus|340635 |BNCC

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Staphylococcus aureus

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	37 ℃; 18-24 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, smooth on the surface, moist in texture, easy to pick up, does not produce pigments, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Storage conditions: 2~8 ℃

No. 340635

Product format: freeze dried, 200ul, or agar slant in 14mm test tube x 1

Validity period: Freeze-dried tube for 24 months, inclined plane for 30 days

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Recovery/subculture:37°C, aerobic, nutrient agar/gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, 18-24hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 20h NB bacterial liquid is turbid and NA strain layer is obvious
colony morphology	size: moderate color: golden yellow/white shape: round edge: full edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: middle bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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