BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC363644 |
| Description | Fastidious Anaerobe Broth (FAB) |
| Composition | Mixed Peptone:15.0g, Yeast Extract Powder:10.0g, Sodium Thioglycolate(C2H3NaO2S):0.5g, NaCl:2.5g, Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, Hemin:0.005g, Vitamin K:0.0005g, NaHCO3:0.4g, Resazurin:0.001g, Agar:0.75g, pH:7.2±0.2(25℃) |
| Growth conditions | 37 ° C; 3-7 days; Strictly anaerobic |
| Subculture procedure | ① Dissolve the Frozen vial tube in a 37 ℃ water bath and rapidly shake for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into the liquid test tube culture medium (the culture medium is deoxygenated 24 hours in advance); ④ Transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G - (red), Bacillus |
| Sharing mode | Public welfare sharing |
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