BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360850 |
| Description | Yeast Extract Glucose Agar |
| Composition | Yeast Extract:5.0g, Glucose:20.0g, Agar:15.0g, pH:6.6±0.2(25℃) |
| Growth conditions | 30°C; 4-5 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Open plates in a biosafety cabinet after surface disinfection; ③ Pipette 1-3 ml sterile water onto plates; scrape bacterial mat from surface using sterile spreader to create suspension; ④ Aspirate suspension with Pasteur pipette and inoculate onto fresh plates, spreading evenly; ⑤ Incubate plates under specified conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5-1 mm; circular shape with neat edges; opaque; grayish-white upper surface; central elevation; smooth, shiny surface; moist texture; easily liftable; G- (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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