BeNa Culture Collection
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| Culture medium | BNCC352255 |
| Description | Streptomyces medium 2 |
| Composition | Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 28°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The strain is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 2-4 mm; irregular shape with jagged edges; opaque, pale color; raised center; rough, wrinkled surface; easily liftable; non-pigmented; Gram-positive (blue-violet); bacillus; Purity: Pure |
| Sharing mode | Public welfare sharing |
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