Trichoderma harzianum|341630 |BNCC

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Trichoderma harzianum

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it apart with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder thoroughly, then dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears.
Growth conditions	28°C; 3-5 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Mycelium is villous, producing yellow spores; culture medium appears pale yellow on the reverse side.
Sharing mode	Public welfare sharing

Trichoderma harzianum

Storage conditions : 2~8 ℃

No. : 341630

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA agar, 5 days. Comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5 days strain layer is obvious
colony morphology	small filamentous fungi have obvious colonies on the integrated PDA medium, white and sparse at the initial stage, producing yellow-green spores, spreading and growing to the edge of the plate. after 5 days, the spores will fill the plate
conclusion	good viability, no abnormal colony morphology, qualified

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