Penicillium hydrophila|120414 |BNCC

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Penicillium pinophilum

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	25-28 ℃; 5-7 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on PDA medium, with a light yellow brown surface and wrinkles on the hyphae. They grow in concentric circles towards the edge of the Agar plate and do not produce spores
Sharing mode	Public welfare sharing

Penicillium pinophilum

Storage conditions: 2~8 ℃

No. 120414

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25-28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: 1.0L potato cooking liquid, 20.0g glucose, 3.0g KH2PO4, 1.5g MgSO4 7H2O, trace amount of vitamin B1, 20.0g agar, pH 6.0±0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 5-7 days plate colony is obvious
colony morphology	small filamentous fungi, with obvious colonies on integrated PDA medium, yellow-green hyphae, dense and vigorous, spread to concentric circles on the edge of the plate and grow without spores.
Conclusion:	good viability, no abnormal colony morphology, qualified

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