White-Yellow Small Pore Fungus|121979 |BNCC

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Antrodiella albocinnamomea Y.C. Dai & Niemelä

Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect the surface of the Agar slant test tube before opening it in a biosafety cabinet; ③ Cut into 0.5×0.5 cm² squares using a sterile inoculating fork and spatula (see appendix); ④ Place the square pieces flat at the center of the Agar plate, on the agar surface; ⑤ Incubate the plates upright under the specified conditions until growth appears.
Growth conditions	25-28°C, 5-7 days, aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungi produce distinct colonies on PDA medium. Mycelium is white with fine surface hairs, growing densely and vigorously in concentric circles toward the Agar plate edges.
Sharing mode	Public welfare sharing

Antrodiella albocinnamomea

Storage conditions : 2~8 ℃

No. : 121979

Product format: agar slant in 14mm test tube

Validity period: growin culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, PDA,5-7 days. PDA: potato boil 1.0L, glucose 20.0g, agar 15.0g, natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 5-7 days plate colony is obvious
colony morphology:	Small filamentous fungi with obvious colonies on PDA medium, white hyphae, fine villi on the surface, dense and vigorous, to plate edge concentric circles spread.
Conclusion:	good viability, no abnormal colony morphology, qualified

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