Italian Penicillium|149066 |BNCC

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Penicillium italicum

Subculture procedure	① Prepare 2 plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Extensive growth, filamentous; white mycelium on the front in early stages, later producing pale green spores; yellow on the reverse; Purity: Pure
Sharing mode	Public welfare sharing

Penicillium italicum

Storage conditions : 2~8 ℃

No. : 149066

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 *7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 ±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Place the plate under the above culture conditions, and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5days strain layer become obvious
colony morphology:	small filamentous fungi have obvious colonies on the comprehensive PDA medium. The mycelium of the plate layer is white in the early stage, and light green spores are produced around and in the center in the later stage. The back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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