BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360797 |
| Description | Brain-heart infusion blood agar |
| Composition | Brain Infusion Powder:5.0g, Heart Infusion Powder:12.5g, Peptone:5.0g, Tryptone:10.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:15.0g, pH:7.4±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5mL sterile water into freeze-dried tube. After complete dissolution of bacterial powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque; pale yellow upper surface; central elevation; smooth texture; moist consistency; easily liftable; G+ (blue-violet); cocci; Purity: Pure |
| Sharing mode | Public welfare sharing |
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