Sodium Nitrite Reducing Oligotrophic Monobacterium|223309 |BNCC

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Stenotrophomonas nitritireducens

Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Growth conditions	30°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat edges; opaque; yellow surface; centrally raised; smooth surface; glossy appearance; moist texture; easily liftable; G- (red); bacillus; purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name：Stenotrophomonas nitritireducens

2. BNCC No.：223309

3.Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, NB medium: peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, distilled water 1.0L,pH7.2±0.2. Sterilization at 121 ℃ for 15min.

2. Atmosphere：aerobic

3. Temperature：30 ℃,24-48h

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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