Streptomyces hygroscopicus|335920 |BNCC

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Streptomyces hygroscopicus

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 2-3 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, circular, irregular edges, opaque, gray white on the front, dark yellow on the back, raised in the middle, rough surface, difficult to pick/cut, no pigment production, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptomyceshygroscopicus(Jensen)WaksmanetHenrici

2. BNCC No.: 335920

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. ISP-2 medium: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 15.0g, distilled water 1.0 L,pH7.3. 121 ℃,15 minutes sterilization.

2, Atmosphere: 25-28 ℃

3. Temperature: 3-5 days

4.Notes:

initiate the recovery, rehydrate the freeze-dried pellets with 0.3ml of sterile water, inoculate two agar slants with all the solution.
To avoid any losses caused by mis-handle, please consult with our technicians if you are not clear about the procedure.

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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