Orange Penicillium|345681 |BNCC

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Penicillium citrinum

Subculture procedure	① Prepare 2 tablets; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce gray green spores, irregular edges, opaque, white front, rough surface, dry texture
Sharing mode	Public welfare sharing

Description

1. Name: Penicillium citrinum

2. BNCC No.: 345681

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, Czapek ' Sagar: sucrose 30.0g,NaNO3 3.0 g,MgSO4·7H2O 0.5 g,KCl 0.5 g,FeSO4·4H2O 0.01 g,K2HPO4 1.0 g, agar 15.0g, distilled water 1.0 L,pH 6.0-6.5.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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