Bacillus viscerosus|186438 |BNCC

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Sphingomonas wittichii

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C; 2-3 days; aerobic;
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 0.5-1mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist
Sharing mode	Public welfare sharing