Bacillus viscerosus|186438 |BNCC

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Sphingomonas wittichii

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Growth conditions	30°C; 2-3 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Size: 0.5-1mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist
Sharing mode	Public welfare sharing

Sphingomonas wittichii

Description

1. BNCC No.: 186438

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1, Nutritional agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [note] adding 5mg MnSO₄ ·H₂O when culturing Bacillus is beneficial to spore production.

2. Atmosphere: aerobic

3. Temperature: 30 ℃,72h

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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