Pseudomonas aeruginosa orange subspecies|231888 |BNCC

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Pseudomonas chlororaphis subsp.aurantiaca

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	30 ℃, 18-24h, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: General Color: Slight Yellow Shape: Circular Edge: Neat Edge, Dry Wet: Moist Smooth: Smooth Transparency: Opaque Rise: Convex, G - (Red), Purity: Pure
Sharing mode	Public welfare sharing

Pseudomonas g chlororaphis subsp.

No.: 231888

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, nutrient gravy medium (NB). Nutritional gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, distilled water 1.0L, pH7.0. 121 ℃,15min.

Recovery steps:
(1)Prepare a flask of NB liquid media.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min).

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h NB bacterial fluid is turbid; The bacterial liquid is marked NA plate with obvious colony
colony morphology:	size: general color: yellowish shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified.

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