Bright white Aspergillus|357832 |BNCC

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Aspergillus candidus

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	24 ° C; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, white mycelium, spreading and growing, with a flocculent texture. In the later stage, the spores are slightly yellow white, and the back of the medium is light yellow
Sharing mode	Public welfare sharing

Aspergillus candidus

Storage conditions : 2~8 ℃

No. : 357832

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) The plate was cultured under the above-mentioned culture conditions, and the bacteria were grown and used.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 7days strain layer is obvious
colony morphology (above)	small filamentous fungi with obvious colonies and white hyphae on PDA medium, spread and grow, the texture is flocculent, the spores are yellowish white at the later stage, and the back of the culture medium is light yellow
Conclusion	good viability, no abnormal colony morphology, qualified

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