Verticillium leptospirum W. Gams|358082 |BNCC

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Verticillium leptobactrum W. Gams

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on PDA medium, white color, dense and flat hyphae, spreading and growing towards the edge of the Agar plate, and light yellow on the back of the medium
Sharing mode	Public welfare sharing

Verticillium leptobactrum

Storage conditions : 2~8 ℃

No. : 358082

Product format： agar slant in 14mm test tube

Validity : growing culture, in 30 dyas

Biosafety level : 1，handle in ultra-clean table or safety cabinet

Growth condition：28°C, aerobic, integrated PDA, 5-7 days.

Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 ·7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

Item	test results
viability :	good viability， in 5days strain layer is obvious
colony morphology:	small filamentous fungi, with obvious colonies on comprehensive PDA medium, White, dense hyphae and low flat, spreading to the edge of the plate, the back of the medium is light yellow.
Conclusion:	good resurrection, no abnormal colony morphology, qualified ;

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