Brettanomyces naardenensis|358379 |BNCC

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Brettanomyces naardenensis

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 48-72h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Milky white Protrusion: Moderately raised Surface: Smooth Texture: Moist and easily pluckable
Sharing mode	Public welfare sharing

Dekkera naardenensis

Storage conditions : 2~8 ℃

No. : 358379

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if the goods are overdue, it will be deemed as good. Dry powder shall not be retained after one use, and can be used after activation for 1~2 generations. please refer to the attached page for the use of inclined plane and bacterial liquid. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions: 28 ℃, aerobic, YM medium, 48-72h. YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 72hours liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
Colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Milk White Protuberance: Middle convex surface: smooth texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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