Bacillus subtilis|359037 |BNCC

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Clostridium beijerinckii

Subculture procedure	①Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving, transfer it back into the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Clostridium beijerinckii

Storage conditions : 2~8 ℃

No. : 359037

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and it can be used after being activated for 1~2 generations. please refer to the attached page for the use of the flat panel. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),24-48h.

Recovery steps:

① Prepare the above flat plate 1-2 block (put it in anaerobic environment for 24 hours in advance);

② Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ suck 0.5mL of sterile water and put it into the freeze-drying tube. after fully dissolving the bacterial powder, put it into the plate 200 μL/piece, coated evenly;

④ Place the plate under the above culture conditions, and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 48hours strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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