Short spined silver fungi|360728 |BNCC

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Cunninghamella blakesleeana

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungus. On PDA medium, colonies are distinct, white, with dense and vigorous mycelium spreading toward the Agar plate edges. The underside of the medium appears pale yellow.
Sharing mode	Public welfare sharing

Cunninghamella blakesleeana

Storage conditions: 2~8 ℃

No. 360728

Product format: agar slant in 14mm test tube

Validity: growing culture in 30days

Biosafety y level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28°C, aerobic, PDA, 3-5 days. PDA: Potato cooking liquid 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

① Prepare 1-2 pieces of PDA plates;

② Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

③ Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

④ Lay flat the small pieces to the center of the agar plate;

⑤ Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3 days, plate colonies are obvious
colony morphology:	small filamentous fungi, with obvious colonies on PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and light yellow on the back of the medium.
conclusion: good viability, no abnormal colony morphology, qualified

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