BeNa Culture Collection
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| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate is about 200 μ L, placed in an anaerobic environment for 24 hours in advance for deoxygenation); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 37 ℃; 48-72 hours; anaerobic |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, easy to pick up, and rod-shaped |
| Sharing mode | Public welfare sharing |