Clostridium perfringens|364172 |BNCC

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Clostridium perfringens

Culture medium	BNCC372404
Description	Reinforced Clostridial Medium (RCM)
Composition	Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃)
Growth conditions	37 ℃; 18-24h; anaerobic;
Subculture procedure	① Prepare one 18mm test tube liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The diameter of the colony is 1-2mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing