Clostridium perfringens|364172 |BNCC

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Clostridium perfringens-BNCC
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Clostridium perfringens

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  • number：364172
  • Form：
    The diameter of the colony is 1-2mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped
Standard strain Quantitative strain DNA extraction
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Clostridium perfringens
Subculture procedure ① Prepare one 18mm test tube liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions 37 ℃; 18-24h; anaerobic;
Storage conditions 2-8 ℃
Safety level 2
morphology The diameter of the colony is 1-2mm, circular, with irregular edges, opaque, yellow on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), rod-shaped
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