Helicobacter pylori|364207 |BNCC

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Helicobacter pylori-BNCC
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  • Helicobacter pylori-BNCC

Helicobacter pylori

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  • number：364207
  • Form：
    The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Vibrio species
Standard strain Quantitative strain DNA extraction
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Helicobacter pylori
Subculture procedure Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Dissolve the frozen storage tube in a 37 ℃ water bath, shake quickly to dissolve, wipe the outer wall of the frozen storage tube with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, pour it into 1-2 agar plates, and apply evenly; ④ Cover the agar surface with sufficient (1-4ml) tryptone soy broth medium to maintain a liquid film throughout the entire cultivation period, and prevent the culture from growing on dry surfaces. ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions 37 ℃; 3-4 days; 5% CO2
Storage conditions -80 ℃
Safety level 2
morphology The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Vibrio species
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